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KELCH-LIKE 9; KLHL9

KELCH-LIKE 9; KLHL9

Alternative titles; symbolsKIAA1354HGNC Approved Gene Symbol: KLHL9Cytogenetic location: 9p21.3 Genomic coordinates (GRCh38): 9:21,329,664-21,335,403 (from N...

Alternative titles; symbols

  • KIAA1354

HGNC Approved Gene Symbol: KLHL9

Cytogenetic location: 9p21.3 Genomic coordinates (GRCh38): 9:21,329,664-21,335,403 (from NCBI)

▼ Cloning and Expression
By sequencing clones obtained from a size-fractionated fetal brain cDNA library, Nagase et al. (2000) cloned KLHL9, which they designated KIAA1354. The transcript contains a repetitive element in its 3-prime end, and the deduced 632-amino acid protein shares significant similarity with the actin-binding protein MAYVEN (KLHL2; 605774). RT-PCR ELISA detected high KLHL9 expression in ovary and moderate expression in all other tissues and specific brain regions examined.

Sumara et al. (2007) stated that KLHL9 contains a highly conserved BACK (BTB and C-terminal Kelch) motif involved in spatially orienting substrates in CUL3 (603136)-based ubiquitin E3 ligases.

▼ Gene Function
Sumara et al. (2007) found that KLHL9, KLHL13 (300655), and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates. The CUL3/KLHL9/KLHL13 complex was the minimum unit required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis. CUL3/KLHL9/KLHL13 acted as an E3 ligase and regulated dynamic localization of the chromosomal passenger protein Aurora B (AURKB; 604970) on mitotic chromosomes and accumulation of Aurora B on the central spindle after anaphase onset. Aurora B directly bound the substrate-recognition domains of KLHL9 and KLHL13 in vitro and coimmunoprecipitated with the CUL3/KLHL9/KLHL13 complex during mitosis. Moreover, Aurora B was ubiquitylated in a CUL3-dependent manner in vivo and by reconstituted CUL3/KLHL9/KLHL13 in vitro. Sumara et al. (2007) concluded that CUL3/KLHL9/KLHL13 is an E3 ligase that controls the dynamic behavior of Aurora B on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.

▼ Gene Structure
Hardy et al. (2004) determined that KLHL9 is an intronless gene that spans 4.0 to 4.5 kb. It has a CpG island at its 5-prime end.

▼ Mapping
By genomic sequence analysis, Hardy et al. (2004) mapped the KLHL9 gene to chromosome 9p21, where it lies near a cluster of interferon genes. They mapped the mouse Klhl9 gene to a region of chromosome 4C4 that shares homology of synteny with human chromosome 9p21.

Hartz (2013) mapped the KLHL9 gene to chromosome 9p21.3 based on an alignment of the KLHL9 sequence (GenBank AB037775) with the genomic sequence (GRCh37).

Tags: 9p21.3