Alternative titles; symbolsmiRNA137MIRN137HGNC Approved Gene Symbol: MIR137Cytogenetic location: 1p21.3 Genomic coordinates (GRCh38): 1:98,046,069-98,046,170...
Alternative titles; symbols
HGNC Approved Gene Symbol: MIR137
Cytogenetic location: 1p21.3 Genomic coordinates (GRCh38): 1:98,046,069-98,046,170 (from NCBI)
MicroRNAs (miRNAs), such as MIR137, are highly conserved small noncoding RNAs. They function by binding to complementary sequences typically located within the 3-prime UTRs of target mRNAs, leading to translational repression or mRNA degradation (summary by Deng et al., 2011).
▼ Cloning and Expression
By searching for miRNA genes in a region of chromosome 1 linked to melanoma, Bemis et al. (2008) identified MIR137.
▼ Gene Function
MITF (156845) is the master regulator of melanocyte cell growth, maturation, apoptosis, and pigmentation. Using a reporter gene assay, Bemis et al. (2008) identified a functional MIR137-binding site in the 3-prime UTR of the MITF transcript. They also identified a variable nucleotide tandem repeat (VNTR) in the 5-prime region of the MIR137 gene that appeared to influence MIR137 expression. In cell lines with 12 VNTRs in this region, the primary MIR137 transcript had an altered secondary structure and was inefficiently processed to mature MIR137, leading to inefficient MITF downregulation. In contrast, cell lines with only 3 VNTRs upstream of MIR137 showed efficient downregulation of MITF expression.
Deng et al. (2011) identified MIR137 as a regulator of CTBP1 (602618) expression. Expression of MIR137 was inversely correlated with that of CTBP1 in melanoma cell lines. The MIR137-binding site in the 3-prime UTR of CTBP1 mRNA is conserved from human to chicken. Pull-down assays revealed that MIR137 interacted with ARGO2 (EIF2C2; 606229) and CTBP1 mRNA. Cotransfection of MIR137 inhibited expression of a reporter gene containing the CTBP1 3-prime UTR, but not when the MIR137-binding site was deleted from the CTBP1 3-prime UTR. Western blot and quantitative RT-PCR analyses showed that MIR137 expression in a melanoma cell line reduced CTBP1 protein levels and increased expression of the CTBP1 target genes E-cadherin (CDH1; 192090) and BAX (600040).
Using methylation-specific PCR, Langevin et al. (2011) found that the promoter region of the MIR137 gene was methylated in 11 (16.4%) of 67 patients with squamous cell carcinoma of the head and neck (275355). Methylation correlated with overall survival, but not with disease-free survival or any other prognostic factors evaluated.
Bemis et al. (2008) reported that the MIR137 gene maps to chromosome 1p22. Gross (2011) mapped the MIR137 gene to chromosome 1p21.3 based on an alignment of the mature MIR137 sequence (UUAUUGCUUAAGAAUACGCGUAG) with the genomic sequence (GRCh37).