Alternative titles; symbolsBRAIN-DERIVED PHOSPHATASE; BDP1HGNC Approved Gene Symbol: PTPN18Cytogenetic location: 2q21.1 Genomic coordinates (GRCh38): 2:130,3...
Alternative titles; symbols
HGNC Approved Gene Symbol: PTPN18
Cytogenetic location: 2q21.1 Genomic coordinates (GRCh38): 2:130,356,011-130,375,404 (from NCBI)
▼ Cloning and Expression
Using a PCR amplification strategy, Kim et al. (1996) isolated nonreceptor protein-tyrosine phosphatase-18 (PTPN18), which they called brain-derived phosphatase (BDP1), from a human fetal brain cDNA library. The PTPN18 cDNA encodes a deduced 459-amino acid protein with no transmembrane domain and a calculated molecular weight of 50 kD. PTPN18 has a PEST motif and shares the greatest homology with members of the PTP-PEST gene family. When transiently expressed in 293 cells, PTPN18 hydrolyzed p-nitrophenylphosphate, confirming that it is a functional protein-tyrosine phosphatase. Northern blot analysis demonstrated that PTPN18 is expressed not only in brain but also in colon and several different tumor-derived cell lines. PTPN18 was found to differentially dephosphorylate autophosphorylated tyrosine kinases which are known to be overexpressed in tumor tissues.
The International Radiation Hybrid Mapping Consortium mapped the PTPN18 gene to chromosome 2 (stSG1724).