Alternative titles; symbolsPPIA-LIKE PROTEIN 4ECYCLOPHILIN A-LIKE PROTEIN 4ECHROMOSOME 1 AMPLIFIED SEQUENCE 2COAS2HGNC Approved Gene Symbol: PPIAL4ECytogenetic l...
Alternative titles; symbols
HGNC Approved Gene Symbol: PPIAL4E
Cytogenetic location: 1q21.1 Genomic coordinates (GRCh38): 1:144,372,874-144,373,633 (from NCBI)
▼ Cloning and Expression
By positional cloning for genes located within a region of chromosome 1 amplified in several cancers, Meza-Zepeda et al. (2002) identified COAS2, and they cloned COAS2 from an osteosarcoma cell line cDNA library. The deduced 164-amino acid protein shares 84% sequence identity with cyclophilin A (PPIA; 123840), including complete conservation of critical residues in the active site and in the cis-proline binding pocket. Northern blot analysis detected COAS2 expression in most normal tissues tested, with highest levels in brain and lowest levels in skeletal muscle. While the COAS2 gene was amplified in all of the liposarcomas studied, elevated expression was detected only in pleomorphic and undifferentiated liposarcomas. COAS2 was also highly expressed in several osteosarcomas and breast cancer cell lines.
Hartz (2011) determined that the amino acid sequence of COAS2 reported by Meza-Zepeda et al. (2002) in their Figure 2 is identical to that of PPIAL4E.
▼ Gene Structure
Meza-Zepeda et al. (2002) determined that the coding sequence of the COAS2 gene is contained within a single exon.
By genomic sequence analysis and FISH, Meza-Zepeda et al. (2002) mapped the COAS2 gene to chromosome 1q21, about 60 kb telomeric from COAS1 (608607).