ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32 FAMILY, MEMBER E; ANP32E

Alternative titles; symbols

• LEUCINE-RICH ACIDIC NUCLEAR PROTEIN-LIKE; LANPL

HGNC Approved Gene Symbol: ANP32E

Cytogenetic location: 1q21.2 Genomic coordinates (GRCh38): 1:150,218,416-150,236,132 (from NCBI)

▼ Cloning and Expression
By searching a fetal brain cDNA library, Jiang et al. (2002) identified ANP32E. The deduced 266-amino acid protein has a predicted molecular mass of 30.4 kD and contains 4 tandem repeats of a 21- to 24-residue leucine-rich region. ANP32E shares 98% amino acid identity with mouse Anp32e and 71% identity with human ANP32A (600832). RT-PCR analysis of 16 adult tissues showed expression in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver, and kidney.

▼ Gene Structure
Jiang et al. (2002) determined that the ANP32E gene contains 7 exons and spans 15.7 kb.

▼ Gene Function
Obri et al. (2014) reported the identification and characterization of the human protein ANP32E as a chaperone specific for H2A.Z (142763) and showed that ANP32E is a member of the presumed H2A.Z histone-exchange complex p400 (606265)/TIP60 (KAT5; 601409). ANP32E interacts with a short region of the docking domain of H2A.Z through a motif termed H2A.Z-interacting domain (ZID).

▼ Biochemical Features
Crystal Structure

Obri et al. (2014) determined the 1.48-angstrom resolution crystal structure of the complex formed between the ANP32E-ZID and the H2A.Z/H2B dimer; the biochemical data supported an underlying molecular mechanism for H2A.Z/H2B eviction from the nucleosome and its stabilization by ANP32E through a specific extension of the H2A.Z carboxy-terminal alpha-helix. Finally, analysis of H2A.Z localization in ANP32E-null cells by chromatin immunoprecipitation, followed by sequencing, showed genome-wide enrichment, redistribution, and accumulation of H2A.Z at specific chromatin control regions, in particular at enhancers and insulators.

▼ Mapping
By genomic sequence analysis, Jiang et al. (2002) mapped the ANP32E gene to chromosome 1q22.