HGNC Approved Gene Symbol: ACOT6Cytogenetic location: 14q24.3 Genomic coordinates (GRCh38): 14:73,610,944-73,619,887 (from NCBI)▼ DescriptionAcyl-CoA thioest...
HGNC Approved Gene Symbol: ACOT6
Cytogenetic location: 14q24.3 Genomic coordinates (GRCh38): 14:73,610,944-73,619,887 (from NCBI)
Acyl-CoA thioesterases (ACOTs; EC 184.108.40.206), such as ACOT6, hydrolyze acyl-CoAs to the free fatty acid and CoA. ACOTs therefore play key roles in maintaining the intracellular ratio between CoA esters of various lipids and free fatty acids (summary by Hunt et al., 2006).
▼ Cloning and Expression
By searching a database for orthologs of mouse Acot genes, followed by PCR amplification, Hunt et al. (2006) cloned human ACOT6. The deduced 207-amino acid protein contains a putative catalytic region, but the protein is N-terminally truncated compared with other ACOTs. Fluorescence-tagged ACOT6 was expressed in the cytosol of transfected human skin fibroblasts.
▼ Gene Structure
Hunt et al. (2006) determined that the ACOT6 gene contains 3 exons, the first of which is noncoding.
By genomic sequence analysis, Hunt et al. (2006) mapped the ACOT6 gene to an 80-kb ACOT gene cluster on chromosome 14q24.3. The order of the genes, from centromere to telomere, is ACOT1 (614313), ACOT2 (609972), ACOT4 (614314), and ACOT6. Hunt et al. (2006) mapped the mouse Acot6 gene to a similar gene cluster on chromosome 12D3, except that the mouse cluster contains 6 Acot genes.