EUKARYOTIC ELONGATION FACTOR 2 LYSINE METHYLTRANSFERASE; EEF2KMT

Alternative titles; symbols

• EEF2 LYSINE METHYLTRANSFERASE
• FAMILY WITH SEQUENCE SIMILARITY 86, MEMBER A; FAM86A

HGNC Approved Gene Symbol: EEF2KMT

Cytogenetic location: 16p13.3 Genomic coordinates (GRCh38): 16:5,084,283-5,097,794 (from NCBI)

▼ Description
Methyltransferases catalyze the transfer of a methyl group from a donor, generally S-adenosyl-L-methionine (AdoMet), to acceptor molecules, including proteins, DNA, RNA, lipids, and small metabolites. FAM86A belongs to a family of protein lysine methyltransferases that methylate nonhistone proteins (Cloutier et al., 2013).

▼ Cloning and Expression
By searching databases for sequences similar to METTL22 (615261), Cloutier et al. (2013) identified FAM86A. The deduced protein is made up predominantly of a Rossmann fold of alternating beta strands and alpha helices, with a conserved AdoMet-binding site near the N terminus. Epitope-tagged FAM86A was predominantly expressed in a diffuse cytoplasmic distribution in transfected HeLa cells.

Davydova et al. (2014) stated that both FAM86A and FAM86B2 (616123) contain 330 amino acids and differ by only 26 point substitutions.

▼ Gene Function
By affinity purification, SDS-PAGE, and mass spectrometry, Cloutier et al. (2013) found that FAM86A expressed in HEK293 cells interacted with FAM86B2 and FAM86C (FAM86C1; 616124).

Davydova et al. (2014) found that FAM86A catalyzed trimethylation of EEF2 (130610) on lys525 (K525), which lies in domain III on the outer surface of a highly conserved alpha helix.

▼ Mapping
Hartz (2013) mapped the FAM86A gene to chromosome 16p13.3 based on an alignment of the FAM86A sequence (GenBank AY037162) with the genomic sequence (GRCh37).