Alternative titles; symbolsARF-GAP, RHO-GAP, ANKYRIN REPEAT, AND PLECKSTRIN HOMOLOGY DOMAINS-CONTAINING PROTEIN 3; ARAP3HGNC Approved Gene Symbol: ARAP3Cytogenet...
Alternative titles; symbols
HGNC Approved Gene Symbol: ARAP3
Cytogenetic location: 5q31.3 Genomic coordinates (GRCh38): 5:141,653,400-141,682,229 (from NCBI)
▼ Cloning and Expression
Krugmann et al. (2002) showed that matrices carrying the tethered homologs of natural phosphoinositides can be used to capture and display multiple phosphoinositide-binding proteins in cell and tissue extracts. They presented the mass spectrometric identification of more than 20 proteins isolated by this method, mostly from leukocyte extracts. One of the novel PtdIns(3,4,5)P3-binding proteins they identified, ARAP3, is homologous to ARAP1 (606646) and ARAP2 (606645). Like ARAP1 and ARAP2, ARAP3 has ARF-GAP (see 103180), RHO-GAP (see 602732), ankyrin repeat (see 605787), RAS (190020)-associating, and 5 pleckstrin (173570) homology (PH) domains. ARAP3 also has a sterile alpha motif (SAM) like that found in ARAP2.
▼ Gene Function
Krugmann et al. (2002) showed that ARAP3 is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated ARF6 (600464) GAP both in vitro and in vivo and that both its ARF-GAP and RHO-GAP domains cooperate in mediating PI3K-dependent rearrangements in the cell cytoskeleton and cell shape.
The lethality of Bacillus anthracis infection is largely due to cellular uptake of its toxin, which consists of a carrier protein, the protective antigen, in combination with either the lethal factor or edema factor moiety. Lu et al. (2004) determined that ARAP3 is a host-cell gene required for anthrax toxicity. Expression of antisense ARAP3 resulted in a toxin-resistant phenotype, and ARAP3-deficient cells showed impaired internalization of the protective antigen.
The International Radiation Hybrid Mapping Consortium mapped the CENTD3 gene to chromosome 5 (WI-11093).