SCY1-LIKE PROTEIN 3; SCYL3

Alternative titles; symbols

• SCY1, S. CEREVISIAE, HOMOLOG OF, 3
• PROTEIN ASSOCIATED WITH THE C-TERMINAL DOMAIN OF EZRIN; PACE1

HGNC Approved Gene Symbol: SCYL3

Cytogenetic location: 1q24.2 Genomic coordinates (GRCh38): 1:169,849,630-169,894,266 (from NCBI)

▼ Cloning and Expression

Using ezrin (VIL2; 123900) as bait in a yeast 2-hybrid screen of a kidney cDNA library, followed by library screening and 5-prime and 3-prime RACE, Sullivan et al. (2003) cloned full-length PACE1. The deduced 688-amino acid protein contains an N-terminal myristoylation consensus sequence, followed by a protein kinase domain, 2 tandemly arrayed HEAT motifs composed of pairs of antiparallel alpha helices, and a C-terminal ezrin-binding domain. They also identified a splice variant encoding a deduced 742-amino acid protein that contains an additional 54 amino acids between the HEAT repeats and the ezrin-binding domain. EST database analysis indicated that PACE1 is expressed in breast carcinoma cells and adult colon. Immunofluorescent staining and confocal microscopy of endogenous and transfected PACE1 detected a fine punctate cytoplasmic staining and enrichment in 2 distinct cellular compartments, lamellipodia and the cytoplasmic face of the Golgi apparatus. In lamellipodia, transfected PACE1 colocalized with endogenous and transfected ezrin. The colocalization of PACE1 with Golgi markers required the N-terminal myristoylation signal.

▼ Gene Function

By yeast 2-hybrid analysis, Sullivan et al. (2003) determined that the C-terminal domain of PACE1 interacts with the C-terminal alpha-helical domain of ezrin. Biochemical assays indicated that PACE1 had associated rather than intrinsic protein kinase activity.

▼ Gene Structure

Sullivan et al. (2003) determined that the PACE1 gene contains 14 exons.

▼ Mapping

By genomic sequence analysis, Sullivan et al. (2003) mapped the PACE1 gene to chromosome 1q23.3-q23.4.